Mostrando títulos en Química

We describe an isothermal, single-reaction method for assembling multiple overlapping DNA molecules by the concerted action of a 5' exonuclease, a DNA polymerase and a DNA ligase. First we recessed DNA fragments, yielding single-stranded DNA overhangs that specifically annealed, and then covalently joined them. This assembly method can be used to seamlessly construct synthetic and natural genes, genetic pathways and entire genomes, and could be a useful molecular engineering tool.DOI:10.1038/NMETH.1318 - 2009

Formation of natural intramolecular triple-helical structures of DNA is still an intriguing research topic in view of the possible involvement of these structures in biological processes. The biochemical and biophysical properties of DNA triplex structures have been extensively studied, and experimental data show that H-DNA is likely to form in vivo and may regulate the expression of various genes. However, direct and unambiguous evidence of the possible biological roles of these structures is yet elusive. This review focuses on the basic facts that are in favor of, or against, the hypothesis ...

In STEM, and particularly in science, many early career researchers find themselves isolated and lacking guidance. There is an enormous need to connect early career scientists with experienced professionals outside their immediate work environment. A new initiative aims to create a supportive community to foster communication between scientists through all stages of their career.https://doi.org/10.1016/j.molmed.2019.08.007 - 2019

Pestmerc is a podcast about and for pest control warriors in the pest control industry. Whether a technician, manager, sales, owner, or product manufacturer Pestmerc is for you. Pestmerc provides pest control news, reviews, how-to’s, and interviews.

Bierklap is een maandelijkse podcast over bier! Met uw gastheren, Jordi Bruynseels & Pieter de Bock Deze maand praten we over volgende items: Intro (00:00:00 - 00:01:36)Maandgasten: William Leclef - Het Anker (00:01:36 - 00:36:35)Beerstyles! Kölsch (00:36:35- 00:49:57)Bier van de maand: Oerknal (00:49:57- 01:01:26)Wist je Datjes: Vol% Bier (01:01:26- 01:14:06)Bier Actua: Rosa Merckx (01:14:06- 01:29:13) #hetanker# #batteliek #goudencarolus #brouwerijdebock #bierklap

Periodically, the podcast that covers all things periods and chemistry! Each week, over this series of six episodes, we here at the University of Oxford will be taking a deep-dive into just how periods have affected us in tutorials, exams, labs and just generally studying at undergrad. We want to talk about how periods can sometimes just get in the bloody way! Periodically is funded by the Royal Society of Chemistry Equality and Diversity fund.

Pneumocystis, a major opportunistic pathogen in patients with a broad range of immunodeficiencies, contains abundant surface proteins encoded by a multicopy gene family, termed the major surface glycoprotein (Msg) gene superfamily. This superfamily has been identified in all Pneumocystis species characterized to date, highlighting its important role in Pneumocystis biology. In this report, through a comprehensive and in-depth characterization of 459 msg genes from 7 Pneumocys- tis species, we demonstrate, for the first time, the phylogeny and evolution of conserved domains in Msg proteins and ...

Slosson reviews the transformation of alchemistry from an obscure and imprecise practice to the science of chemistry. Along the way, he explains how the modern industrial world now relies on fertilizers, explosives, textile materials, polymers and metals.By exploring the properties of a once undervalued element, the high strength of vanadium steel made the Ford car possible. Another element, cerium, appears in butane lighters and was once seen as a threat to the match industry in France. In his chapter on oils, Slosson reviews the development of hydrogenated oils, especially during WWII, in ...

Myeloid derived suppressors cells (MDSC) play major roles in regulating immune homeostasis and immune responses in many conditions, including cancer. MDSC interact with cancer cells within the tumor microenvironment (TME) with direct and indirect mechanisms: production of soluble factors and cytokines, expression of surface inhibitory molecules, metabolic rewiring and exosome release. The two-way relationship between MDSC and tumor cells results in immune evasion and cancer outgrowth. In multiple myeloma (MM), MDSC play a major role in creating protumoral TME conditions. In this minireview, we...

Early career researchers face uncertainties with respect to their job prospects due to dwindling job markets, decreased availability of funding and undefined career paths. As basic researchers and clinicians tend to have different approaches to scientific problems, there are many advantages from successful collaborations between them. Here, we discuss how collaborations between basic and clinical scientists should be promoted early in their careers. To achieve this, researchers, both basic and clinical, must be proactive during their training and early stages of their careers. Mentors can ...

DNA has emerged as an attractive medium for archival data storage due to its durability and high information density. Scalable parallel random access to information is a desirable property of any storage system. For DNA-based storage systems, however, this still needs to be robustly established. Here we report on a thermoconfined polymerase chain reaction, which enables multiplexed, repeated random access to compartmentalized DNA files. The strategy is based on localizing biotin-functionalized oligonucleotides inside thermoresponsive, semipermeable microcapsules. At low temperatures, ...

Induced pluripotent stem cells (iPSCs) enable the generation of previously unattainable, scalable quantities of disease- relevant tissues from patients suffering from essentially any genetic disorder. This cellular material has proven instrumental for drug screening efforts on these disorders, and has facilitated the identification of novel therapeutics for patients. Here we will review the foundational technologies that have enabled iPSCs, the power and limitations of iPSC-based compound screens along with screening guidelines, and recent examples of screening efforts. Additionally we will ...

Kuudennen osan teema on iso ja merkittävä – vastuullisuus. Vieraina mukana keskustelussa ovat Bernerin viestintä- ja vastuullisuuspäällikkö Maria Nymander ja Metsä Boardin kestävän kehityksen päällikö Sari Koski. Kevennysosiona jälleen Wabi-sabi.

Improving the reproducibility of neuroscience research is of great concern, especially to early-career researchers (ECRs). Here I outline the potential costs for ECRs in adopting practices to improve reproducibility. I highlight the ways in which ECRs can achieve their career goals while doing better science and the need for established researchers to support them in these efforts.https://doi.org/10.1016/j.neuron.2018.11.030 - 2019

Precipitation of DNA is performed frequently in molecular biology laboratories for the purpose of purification and concentration of samples and also for transfer of DNA into cells. Metal ions are used to facilitate these processes, though their precise functions are not well characterized. In the current study we have investigated the precipitation of double-stranded DNA by group 1 and group 2 metal ions. Double-stranded DNAs were not sedimented efficiently by metals alone, even at high concentrations. Increasing the pH to 11 or higher caused strong DNA precipitation in the presence of the ...

A new method for determining nucleotide sequences in DNA is described. It is similar to the "plus and minus" method but makes use of the 2',3 '- dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage phiX174 and is more rapid and more accurate than either the plus or the minus method.doi: 10.1073/pnas.74.12.5463. - 1977

High dropout rates, delay, and dissatisfaction among PhD students are common problems in doctoral education. Research shows that many different factors are associated with doctoral success, but these factors have not often been studied simultaneously. Moreover, characteristics of the PhD project are mostly neglected. In this study, we investigate which supervision, psychosocial, and project characteristics are related to satisfaction, progress, and quit intentions in a sample of 839 PhD candidates at a university in the Netherlands. Results of regression analyses show that experienced workload...

Come explore the exciting world of science from chemistry to biology, paleontology to astrophysics. Get reconnected with the subject of imagination and wonder and take a deeper dive into some of your favorite branches.

Inspiring stories and advice from dPCR gene-iuses.

Chromatin is the complex of DNA and proteins in which the genetic material is packaged inside the cells of organisms with nuclei. Chromatin structure is dynamic and exerts profound control over gene expression and other fundamental cellular processes. Changes in its structure can be inherited by the next generation, independent of the DNA sequence itself.doi:10.1038/nature01411 - 2003