The Good, the Bad, and the Post Unmixing Compensation

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Here it is! The debate that will settle this business of post acquisition adjustment of your unmixing matrix. Is it an appropriate thing to do? Or will applying manual compensation to your data send you to the 9th circle of hell (the one with the puppy stuck in ice)? So fine, my awesome guests Florian Mair and Rui Gardner do not come up with the final answer during this very insightful discussion. But I think the discussion will get us flow cytometry nerds look at the issue in a different way, and offers valuable insights for anyone struggling with incomplete correction of their spectral overlap!

Two notable shoutouts:

• Colibri Cytometry blog: https://www.colibri-cytometry.com/blog
  
  
• Flow Discord Channel: / discord
  
  

The Panel Design Workshop in Zurich will be from January 26-30 2026. Stay tuned for details and registration!