E9: Single Cell Sequencing

Chapters:00:00 Gambling15:00 Bubbles30:00 Holes57:30 One more thing1:07:00 Finale1:08:00 Finale of season 1!Eric Chow explaining single cell sequencing: https://www.youtube.com/watch?v=k9VFNLLQP8cOverview of things to consider: https://www.youtube.com/watch?v=VWUPKrnRvoQHow to analyze single cell data: https://www.youtube.com/watch?v=5HBzgsz8qykA very good single cell analysis toolkit: https://satijalab.org/seurat/Explainer for the rough workflow:https://www.technologynetworks.com/genomics/articles/understanding-single-cell-sequencing-how-it-works-and-its-applications-357578Quick explainer on single cell sequencing:https://www.the-scientist.com/single-cell-sequencing-in-a-nutshell-71048A method-overview paper: https://www.nature.com/articles/s12276-020-00499-2Comparing BD Parse and 10xGenomics: https://pubmed.ncbi.nlm.nih.gov/33414681/Pseudotime analysis: https://bioconductor.org/books/3.14/OSCA.advanced/trajectory-analysis.htmlSince 2018, West German Genome Center (https://wggc.de/) operates as the next generation sequencing facility and supports research in genomics & transcriptomics by providing expertise and sequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/) - network of NGS core facilities in Germany.Listen to our podcast and send your feedback to ( podcast[dot]explain[at]gmail[dot]com ).The Explain Podcast is recorded at the Medienlabor at Heinrich-Heine University of Düsseldorf (https://medienlab.phil.hhu.de/). Our podcast is an outreach activity within the national genomics facility "West German Genome Center" funded by the DFG (https://www.dfg.de/) (No. 407493903).PS: I do not know how in the Parse approach the cells are treated to let the ligase and DNA enter each cell, but do not let RNA flow out of the cell at the same time. -DRLicense NoticeThis podcast including all episodes, unless otherwise noted, is licensed under a ⁠⁠⁠Creative Commons Attribution 4.0 International License⁠⁠⁠.You are free to share, remix, and use this content as long as you give appropriate credit, provide a link to the license, and indicate if changes were made.Attribution:Podcast Title: Explain PodcastCreators: Iuliia Novoselova, Daniel RickertWebsite: ⁠⁠⁠https://ngs-cn.de/explain-podcast/⁠